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Antibody used in WB on Human Placenta at 0.625 ug/ml.
Antibody used in WB on Human 293T at: 1:1000 (Lane 1: 10ug hFXYD5 transfected 293T lysate, Lane 2: 10ug 293T lysate).
Antibody used in WB on Human Placenta at 0.625 ug/ml.

FXYD5 Antibody

CATALOG NUMBER: 28-718

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human FXYD5.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
FXYD5 antibody can be used for detection of FXYD5 by ELISA at 1:312500. FXYD5 antibody can be used for detection of FXYD5 by western blot at 0.625 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
Cat. No. 1309 - Human Placenta Lysate
Predicted Molecular Weight:
20 kDa, 19 kDa
Purification:
Antibody is purified by protein A chromatography method.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store FXYD5 antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
FXYD5
Additional Names:
FXYD5, RIC, IWU1, KCT1, OIT2, DYSAD, HSPC113, PRO6241
Protein Accession Number:
NP_659003
Protein Gi Number:
47778938
Ncbi Gene Id Number:
53827
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
FXYD5 is a member of a family of small membrane proteins that share a 35-amino acid signature sequence domain, beginning with the sequence PFXYD and containing 7 invariant and 6 highly conserved amino acids. The approved human gene nomenclature for the family is FXYD-domain containing ion transport regulator. Mouse FXYD5 has been termed RIon Channel (Related to Ion Channel). FXYD2, also known as the gamma subunit of the Na,K-ATPase, regulates the properties of that enzyme. FXYD1 (phospholemman), FXYD2 (gamma), FXYD3 (MAT-8), FXYD4 (CHIF), and FXYD5 (RIon Channel) have been shown to induce channel activity in experimental expression systems. Transmembrane topology has been established for two family members (FXYD1 and FXYD2), with the N-terminus extracellular and the C-terminus on the cytoplasmic side of the membrane. This gene product, FXYD5, has not been characterized as a protein.
Background Reference 1:
Batistatou, A., et al., (2005) Br. J. Cancer 93 (12), 1382-1387.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

28-718

List Size:
100 ul

List Price:

$473.00

Datasheet
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