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43-248 (0.2ug/ml) staining of MOLT4 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
43-248 (0.2ug/ml) staining of MOLT4 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

FYB Antibody

CATALOG NUMBER: 43-248

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Dog, Cow
Immunogen:
The immunogen for this antibody is: C-SEEKPDKEPKP
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:2000.Western Blot:Approx 90kDa band observed in lysates of cell line MOLT4 (calculated MW of 90.6kDa according to NP_001456.3). Recommended concentration: 0.2-0.6ug/ml.
Specificity:
This antibody is expected to recognise all reported isoforms (NP_001456.3; NP_955367.1; NP_001230022.1).
Predicted Molecular Weight:
Approx 90kDa
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
FYB
Additional Names:
FYB, FYN binding protein (FYB-120/130) , ADAP, PRO0823, SLAP-130, FYN-binding protein (FYB-120/130), adhesion and degranulation-promoting adaptor protein
Protein Accession Number:
NP_001456.3,NP_955367.1,NP_001230022.1
Protein Gi Number:
42476118
Ncbi Gene Id Number:
2533
Background Reference 1:
Medeiros RB, Burbach BJ, Mueller KL, Srivastava R, Moon JJ, Highfill S, Peterson EJ, Shimizu Y.Regulation of NF-kappaB activation in T cells via association of the adapter proteins ADAP and CARMA1.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

43-248

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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