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Western blot analysis of extracts of various cell lines, using FYN antibody (13-026) at 1:500 dilution.<br/>Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 60s.
Western blot analysis of extracts of various cell lines, using FYN antibody (13-026) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 60s.

FYN Antibody [5F3B6]

CATALOG NUMBER: 13-026

Clonality:
Monoclonal
Tested Applications:
ICC, WB
Host Species:
Mouse
Species Reactivity:
Human, Monkey, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
Human, Monkey, Mouse, Rat
Immunogen:
Recombinant protein of human FYN
Conjugate:
Unconjugated
Tested Applications:
ICC, WB
Application Note:
WB: 1:200 - 1:1000
ICC: 1:50 - 1:100
Positive Control 1:
HeLa
Positive Control 2:
Jurkat
Positive Control 3:
SW620
Positive Control 4:
HT-1080
Positive Control 5:
Mouse lung
Positive Control 6:
Mouse heart
Predicted Molecular Weight:
Observed: 60kDa
Purification:
Affinity purification
Clonality:
Monoclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
FYN
Additional Names:
FYN, SLK, SYN, MGC45350, FYN oncogene related to SRC, FGR, YES, src-like kinase, c-syn protooncogene, tyrosine kinase p59fyn(T), src/yes-related novel gene, OKT3-induced calcium influx regulator, proto-oncogene tyrosine-protein kinase fyn, p59-FYN
Ncbi Gene Id Number:
2534
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene is a member of the protein-tyrosine kinase oncogene family. It encodes a membrane-associated tyrosine kinase that has been implicated in the control of cell growth. The protein associates with the p85 subunit of phosphatidylinositol 3-kinase and interacts with the fyn-binding protein. Alternatively spliced transcript variants encoding distinct isoforms exist.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

13-026

List Size:
50 uL

List Price:

$335.00

Out of stock

Datasheet
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