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In a sandwich ELISA (assuming 100μl/well), a concentration of 2.0-4.0 μg/ml of this antibody will detect at least 100pg/ml of Recombinant Human G-CSF when used with ProSci's Biotinylated Antigen Affinity Purified anti-Human G-CSF as the detection antibody, at a concentration of approximately 0.5- 1.0 μg/ml.
To detect Human G-CSF by Western Blot analysis, this antibody can be used at a concentration of 0.20-0.40 μg/ml. When used in conjunction with compatible secondary reagents the detection limit for Recombinant Human G-CSF is 1.5-2.0 ng/lane under non-reducing conditions, and 7.5-10.0 ng/lane under reducing conditions.
In a sandwich ELISA (assuming 100μl/well), a concentration of 2.0-4.0 μg/ml of this antibody will detect at least 100pg/ml of Recombinant Human G-CSF when used with ProSci's Biotinylated Antigen Affinity Purified anti-Human G-CSF as the detection antibody, at a concentration of approximately 0.5- 1.0 μg/ml.

G-CSF Antibody

CATALOG NUMBER: 38-310

Clonality:
Monoclonal
Tested Applications:
ELISA, WB
Host Species:
Mouse
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
Human
Immunogen:
Produced in BALB/c mice immunized with highly pure Recombinant Human G-CSF. Anti-Human G-CSF-specific antibody was purified from cell culture by Protein A affinity chromatography.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Sandwich ELISA: In a sandwich ELISA (assuming 100μl/well), a concentration of 2.0-4.0 μg/ml of this antibody will detect at least 100pg/ml of Recombinant Human G-CSF when used with ProSci’s Biotinylated Antigen Affinity Purified anti-Human G-CSF as the detection antibody, at a concentration of approximately 0.5- 1.0 μg/ml.
Western Blot
To detect Human G-CSF by Western Blot analysis, this antibody can be used at a concentration of 0.20-0.40 μg/ml. When used in conjunction with compatible secondary reagents the detection limit for Recombinant Human G-CSF is 1.5-2.0 ng/lane under non-reducing conditions, and 7.5-10.0 ng/lane under reducing conditions.
Purification:
G-CSF-specific antibody was purified from cell culture by Protein A affinity chromatography
Clonality:
Monoclonal
physical-state:
Lyophilized
Concentration:
batch dependent
Storage Conditions:
Antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
Ncbi Official Symbol:
CSF3
Additional Names:
Granulocyte Colony-Stimulating Factor, CSF-3, MGI-1G, GM-CSFbeta, pluripoietin
Protein Accession Number:
P09919
Protein Gi Number:
P09919.1
Ncbi Gene Id Number:
1440
User Note:
Centrifuge vial prior to opening.
Background:
G-CSF is a hematopoietic growth factor that stimulates the development of committed progenitor cells to neutrophils and enhances the functional activities of the mature end-cell. It is produced in response to specific stimulation by a variety of cells, including macrophages, fibroblasts, endothelial cells and bone marrow stroma. G-CSF is being used clinically to facilitate hematopoietic recovery after bone marrow transplantation. Human and murine G-CSF are cross-species reactive. Recombinant Human G-CSF is an 18.7 kDa protein consisting of 174 amino acid residues.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

38-310

List Size:
0.5 mg

List Price:

$366.00

Datasheet
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