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Western blot analysis in A549 cell line lysates (35ug/lane).
Western blot analysis in A549 cell line lysates (35ug/lane).

G2E3 Antibody

CATALOG NUMBER: 60-196

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Monkey
Immunogen:
This G2E3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 226-252 amino acids from the Central region of human G2E3.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
81 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
G2E3
Additional Names:
G2/M phase-specific E3 ubiquitin-protein ligase, 632-, G2E3, KIAA1333
Protein Accession Number:
Q7L622
Protein Gi Number:
74738611
Ncbi Gene Id Number:
55632
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Essential in early embryonic development to prevent apoptotic death.
Background Reference 1:
Brooks, W.S., et al. J. Biol. Chem. 283(32):22304-22315(2008)
Background Reference 2:
Brooks, W.S., et al. Exp. Cell Res. 313(4):665-676(2007)
Background Reference 3:
Lehner, B., et al. Genomics 83(1):153-167(2004)
Background Reference 4:
Crawford, D.F., et al. J. Biol. Chem. 276(40):37166-37177(2001)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

60-196

List Size:
400 ul

List Price:

$495.00

Datasheet
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