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Antibody used in WB on Human HepG2 at 2.5 ug/ml.
Antibody used in WB on HepG2 at 2.5 ug/ml (Lane A: Primary Antibody and Lane B: Primary Antibody + Blocking Peptide ).
Antibody used in WB on Human HepG2 at 2.5 ug/ml.

G3BP Antibody

CATALOG NUMBER: 28-725

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Dog, Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Dog, Human, Mouse
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human G3BP.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
G3BP antibody can be used for detection of G3BP by ELISA at 1:312500. G3BP antibody can be used for detection of G3BP by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
Cat. No. 1211 - HepG2 Cell Lysate
Predicted Molecular Weight:
51 kDa, 52 kDa
Purification:
Antibody is purified by protein A chromatography method.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store G3BP antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
G3BP1
Additional Names:
G3BP, G3BP, HDH-VIII
Protein Accession Number:
NP_005745
Protein Gi Number:
5031703
Ncbi Gene Id Number:
10146
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
G3BP is one of the DNA-unwinding enzymes which prefers partially unwound 3'-tailed substrates and can also unwind partial RNA/DNA and RNA/RNA duplexes in an ATP-dependent fashion. This enzyme is a member of the heterogeneous nuclear RNA-binding proteins and is also an element of the Ras signal transduction pathway. It binds specifically to the Ras-GTPase-activating protein by associating with its SH3 domain. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined.
Background Reference 1:
Kociok, N., et al., (1999) J. Cell. Biochem. 74 (2), 194-201.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

28-725

List Size:
100 ul

List Price:

$473.00

Datasheet
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