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Western blot analysis in Jurkat cell line lysates (35ug/lane).
G3BP1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western blot analysis in Jurkat cell line lysates (35ug/lane).

G3BP1 Antibody

CATALOG NUMBER: 57-271

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Mouse
Immunogen:
This G3BP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 401-430 amino acids from the C-terminal region of human G3BP1.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
52 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
G3BP1
Additional Names:
Ras GTPase-activating protein-binding protein 1, G3BP-1, ATP-dependent DNA helicase VIII, hDH VIII, GAP SH3 domain-binding protein 1, G3BP1, G3BP
Protein Accession Number:
Q13283
Protein Gi Number:
14916572
Ncbi Gene Id Number:
10146
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes one of the DNA-unwinding enzymes whichprefers partially unwound 3'-tailed substrates and can also unwindpartial RNA/DNA and RNA/RNA duplexes in an ATP-dependent fashion.This enzyme is a member of the heterogeneous nuclear RNA-bindingproteins and is also an element of the Ras signal transductionpathway. It binds specifically to the Ras-GTPase-activating proteinby associating with its SH3 domain. Several alternatively splicedtranscript variants of this gene have been described, but thefull-length nature of some of these variants has not beendetermined.
Background Reference 1:
Gao, X., et al. FEBS Lett. 584(16):3525-3532(2010)
Background Reference 2:
Ortega, A.D., et al. J. Cell. Sci. 123 (PT 16), 2685-2696 (2010) :
Background Reference 3:
Hinton, S.D., et al. Biochem. J. 427(3):349-357(2010)
Background Reference 4:
Shim, J.H., et al. Cancer Prev Res (Phila) 3(5):670-679(2010)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

57-271

List Size:
400 ul

List Price:

$495.00

Datasheet
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