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Western Blot at 1:1000 dilution + HepG2 whole cell lysate Lysates/proteins at 20 ug per lane.
Western blot analysis of G6PC Antibody in K562 cell line lysates (35ug/lane)
G6PC Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western Blot at 1:1000 dilution + HepG2 whole cell lysate Lysates/proteins at 20 ug per lane.

G6PC Antibody

CATALOG NUMBER: 61-842

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This G6PC antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 123-149 amino acids from the Central region of human G6PC.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
40 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
G6PC
Additional Names:
Glucose-6-phosphatase, G-6-Pase, G6Pase, Glucose-6-phosphatase alpha, G6Pase-alpha, G6PC, G6PT
Protein Accession Number:
P35575
Protein Gi Number:
206729864
Ncbi Gene Id Number:
2538
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Glucose-6-phosphatase is an integral membrane protein of the endoplasmic reticulum that catalyzes the hydrolysis of D-glucose 6-phosphate to D-glucose and orthophosphate. It is a key enzyme in glucose homeostasis, functioning in gluconeogenesis and glycogenolysis. Defects in the enzyme cause glycogen storage disease type I.
Background Reference 1:
Tu, E., et al. Hum. Pathol. 41(3):392-400(2010)
Background Reference 2:
Samuel, V.T., et al. Proc. Natl. Acad. Sci. U.S.A. 106(29):12121-12126(2009)
Background Reference 3:
Hu, C., et al. Diabetologia 52(3):451-456(2009)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

61-842

List Size:
400 ul

List Price:

$495.00

Datasheet
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