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Western blot analysis of extracts of various cell lines, using G6PC3 antibody (16-504) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 5min.
Western blot analysis of extracts of various cell lines, using G6PC3 antibody (16-504) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 5min.

G6PC3 Antibody

CATALOG NUMBER: 16-504

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 50-150 of human G6PC3 (NP_612396.1).
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
WB: 1:500 - 1:2000
Positive Control 1:
K-562
Positive Control 2:
HeLa
Positive Control 3:
293T
Positive Control 4:
Mouse kidney
Positive Control 5:
Mouse heart
Predicted Molecular Weight:
Observed: 37kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
G6PC3
Additional Names:
SCN4, UGRP, glucose-6-phosphatase 3, G-6-Pase 3, G6Pase 3, G6Pase-beta, glucose 6 phosphatase, catalytic, 3, ubiquitous glucose-6-phosphatase catalytic subunit-related protein, ubiquitously expressed G6Pase catalytic subunit-related protein
Ncbi Gene Id Number:
92579
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes the catalytic subunit of glucose-6-phosphatase (G6Pase). G6Pase is located in the endoplasmic reticulum (ER) and catalyzes the hydrolysis of glucose-6-phosphate to glucose and phosphate in the last step of the gluconeogenic and glycogenolytic pathways. Mutations in this gene result in autosomal recessive severe congenital neutropenia. Alternative splicing results in multiple transcript variants.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

16-504

List Size:
100 uL

List Price:

$436.00

Out of stock

Datasheet
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