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Western blot analysis of G6PD Antibody in K562,MCF-7 cell line lysates (35ug/lane)
G6PD Antibody IHC analysis in formalin fixed and paraffin embedded testis followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of G6PD Antibody in K562,MCF-7 cell line lysates (35ug/lane)

G6PD Antibody

CATALOG NUMBER: 61-790

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This G6PD antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 297-326 amino acids from the Central region of human G6PD.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
59 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
G6PD
Additional Names:
Glucose-6-phosphate 1-dehydrogenase, G6PD, G6PD
Protein Accession Number:
P11413
Protein Gi Number:
116242483
Ncbi Gene Id Number:
2539
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
G6PD encodes glucose-6-phosphate dehydrogenase. This protein is a cytosolic enzyme encoded by a housekeeping X-linked gene whose main function is to produce NADPH, a key electron donor in the defense against oxidizing agents and in reductive biosynthetic reactions. G6PD is remarkable for its genetic diversity. Many variants of G6PD, mostly produced from missense mutations, have been described with wide ranging levels of enzyme activity and associated clinical symptoms. G6PD deficiency may cause neonatal jaundice, acute hemolysis, or severe chronic non-spherocytic hemolytic anemia.
Background Reference 1:
Louicharoen, C., et al. Science 326(5959):1546-1549(2009)
Background Reference 2:
Zhong, D.N., et al. Zhongguo Dang Dai Er Ke Za Zhi 11(12):970-972(2009)
Background Reference 3:
Tiono, A.B., et al. Am. J. Trop. Med. Hyg. 81(6):969-978(2009)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

61-790

List Size:
400 ul

List Price:

$495.00

Datasheet
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