Western blot analysis of extracts of various cell lines, using GAB1 antibody (22-081) at 1:500 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 80s.

Immunohistochemistry of paraffin-embedded rat brain using GAB1 antibody (22-081) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human colon carcinoma using GAB1 antibody (22-081) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human gastric cancer using GAB1 antibody (22-081) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse spinal cord using GAB1 antibody (22-081) at dilution of 1:100 (40x lens).

Western blot analysis of extracts of various cell lines, using GAB1 antibody (22-081) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 80s.

GAB1 Antibody

CATALOG NUMBER: 22-081

Clonality:

Polyclonal

Tested Applications:

IF, IHC, WB

Host Species:

Rabbit

Species Reactivity:

Human, Mouse, Rat

Conjugate:

Unconjugated

Specifications

Host Species:

Rabbit

Species Reactivity:

Human, Mouse, Rat

Immunogen:

Recombinant fusion protein containing a sequence corresponding to amino acids 485-724 of human GAB1 (NP_997006.1).

Conjugate:

Unconjugated

Tested Applications:

IF, IHC, WB

Application Note:

WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:20 - 1:100

Positive Control 1:

293T

Positive Control 2:

HepG2

Positive Control 3:

U-251MG

Positive Control 4:

SH-SY5Y

Positive Control 5:

Mouse heart

Positive Control 6:

Mouse kidney

Predicted Molecular Weight:

Observed: 76kDa

Advanced Validation

Properties

Purification:

Affinity purification

Clonality:

Polyclonal

Isotype:

IgG

Physical State:

Liquid

Buffer:

PBS with 0.09% Sodium azide, 50% glycerol, pH7.3.

Concentration:

batch dependent

Storage Conditions:

Store at -20°C. Avoid freeze / thaw cycles.

Additional Info

Ncbi Official Symbol:

GAB1

Additional Names:

ADDA, Growth factor receptor bound protein 2-associated protein 1

Ncbi Gene Id Number:

2549

User Note:

Optimal dilutions for each application to be determined by the researcher.

Background

Background:

The protein encoded by this gene is a member of the IRS1-like multisubstrate docking protein family. It is an important mediator of branching tubulogenesis and plays a central role in cellular growth response, transformation and apoptosis. Two transcript variants encoding different isoforms have been found for this gene.

Citations

Set Contents

Disclaimer

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:

Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

22-081

List Size:

100 uL

List Price:

$436.00

Datasheet

Shipping Info

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$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

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GAB1 Antibody

Clonality:

Tested Applications:

Host Species:

Species Reactivity:

Conjugate:

Host Species:

Species Reactivity:

Immunogen:

Conjugate:

Tested Applications:

Application Note:

Positive Control 1:

Positive Control 2:

Positive Control 3:

Positive Control 4:

Positive Control 5:

Positive Control 6:

Predicted Molecular Weight:

Purification:

Clonality:

Isotype:

Physical State:

Buffer:

Concentration:

Storage Conditions:

Ncbi Official Symbol:

Additional Names:

Ncbi Gene Id Number:

User Note:

Background:

Disclaimer:

List Size:

Shipping Info

Customize your order

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