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Western blot analysis of GABARAP in HeLa cell lysate with GABARAP antibody at 1 μg/ml.
Immunofluorescence of GABARAP in human brain tissue with GABARAP antibody at 20 μg/ml.
Immunohistochemistry of GABARAP in mouse brain tissue with GABARAP Antibody at 5 μg/mL.
Immunofluorescence of GABARAP in mouse brain tissue with GABARAP Antibody at 20 μg/mL. <br><br>Green: GABARAP antibody (7359) <br> Red: Phylloidin staining<br> Blue: DAPI staining
Western blot analysis of GABARAP in HeLa cell lysate with GABARAP antibody at 1 μg/ml.
Trial Size

GABARAP Antibody

CATALOG NUMBER: 7359

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Rabbit: (100%), Bovine: (100%)
Immunogen:
GABARAP antibody was raised against a 19 amino acid peptide near the amino terminus of human GABARAP.
The immunogen is located within the first 50 amino acids of GABARAP.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
GABARAP antibody can be used for detection of GABARAP by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in human and mouse samples. All other applications and species not yet tested.
Specificity:
Multiple isoforms of GABARAP exist due to alternative splicing events.
Positive Control 1:
Cat. No. 1201 - HeLa Cell Lysate
Positive Control 2:
Cat. No. 10-301 - Human Brain Tissue Slide
Predicted Molecular Weight:
Predicted: 13kDa
Observed: 15 kDa
Purification:
GABARAP Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
GABARAP Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
GABARAP antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Ncbi Official Symbol:
GABARAP
Additional Names:
GABARAP Antibody: MM46, ATG8A, GABARAP-a, FLC3B, HT004, Gamma-aminobutyric acid receptor-associated protein, MM46
Protein Accession Number:
NP_009209
Protein Gi Number:
6005764
Ncbi Gene Id Number:
11337
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
GABARAP Antibody: Gamma-aminobutyric acid (GABA) is the main inhibitory transmitter by increasing a Cl-conductance that inhibits neuronal firing in the central nervous system. It has been shown to activate both ionotropic (GABAA) and metabotropic (GABAB) receptors as well as a third class of receptors called GABAC. GABARAP (GABAA receptor-associated protein) links GABAA receptors to the cytoskeleton and may play a role in intracellular transport of GABAA receptors and its interaction with the cytoskeleton. GABARAP belongs to the MAP1 or ATG8 like family and recent studies show that MAPK15/ERK8 is acting through interaction with ATG8 family proteins to regulate autophagy.
Background Reference 1:
Cherubini E, Gaiarsa JL, and Ben-Ari Y. GABA: an excitatory transmitter in early postnatal life. Trends Neurosci.1991; 14:515-19.
Background Reference 2:
Dirkx R Jr, Thomas A, Li L, et al. Targeting of the 67 kDa isoform of glutamic acid decarboxylase to intracellular organelles is mediated by its interaction with the NH2-terminal region of the 65 kDa isoform of glutamic acid decarboxylase. J. Biol. Chem. 1995; 270:2241-6.
Background Reference 3:
Wang H, Bedford FK, Brandon NJ, et al. GABA(A)-receptor-associated protein links GABAA receptors and the cytoskeleton. Nature 1999; 397:69-72.
Background Reference 4:
Colecchia D, Strambi A, Sanzone S, et al. MAPK15/ERK8 stimulates autophagy by interacting with LC3 and GABARAP proteins. Autophagy 2012 Sep 4;8(12).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

7359

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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