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Western blot analysis of various lysates using GABARAP Rabbit mAb at 1:1000 dilution. 293T、NIH/3T3 and C6 cells were treated with Chloroquine (50 μM) at 37℃ for 20 hours. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 25μg per lane. | Blocking buffer: 3% BSA. | Detection: ECL Basic Kit. | Exposure time: 10s.
Western blot analysis of various lysates using GABARAP Rabbit mAb at 1:1000 dilution. 293T、NIH/3T3 and C6 cells were treated with Chloroquine (50 μM) at 37℃ for 20 hours. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 25μg per lane. | Blocking buffer: 3% BSA. | Detection: ECL Basic Kit. | Exposure time: 10s.

GABARAP Rabbit Recombinant Antibody (83-882)

CATALOG NUMBER: 83-882

Clonality:
Monoclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
WB,1:500 - 1:2000, ELISA,Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Positive Control 1:
293T treated with Chloroquine,NIH/3T3 treated with Chloroquine,C6 treated with Chloroquine
Predicted Molecular Weight:
14kDa
purification:
Affinity purification
Clonality:
Monoclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Concentration:
Batch dependent
Storage Conditions:
Store at -20℃. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
GABARAP
Additional Names:
MM46; ATG8A; GABARAP-a; GABARAP
Protein Accession Number:
O95166
Ncbi Gene Id Number:
11337
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Gamma-aminobutyric acid A receptors [GABA(A) receptors] are ligand-gated chloride channels that mediate inhibitory neurotransmission. This gene encodes GABA(A) receptor-associated protein, which is highly positively charged in its N-terminus and shares sequence similarity with light chain-3 of microtubule-associated proteins 1A and 1B. This protein clusters neurotransmitter receptors by mediating interaction with the cytoskeleton.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

83-882

List Size:
100 uL

List Price:

$477.00

Datasheet
Shipping Info

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