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Western blot analysis of GABARAPL2 in human brain tissue lysate with GABARAPL2 antibody at (A) 1 and (B) 2 μg/ml.
Immunohistochemistry of GABARAPL2 in rat brain tissue with GABARAPL2 antibody at 5 μg/mL.
Immunofluorescence of GABARAPL2 in rat brain tissue with GABARAPL2 antibody at 20 μg/mL.
Immunohistochemistry of GABARAPL2 in mouse brain tissue with GABARAPL2 Antibody at 5 μg/mL.
Immunofluorescence of GABARAPL2 in mouse brain tissue with GABARAPL2 Antibody at 20 μg/mL.<br><br>Green: GABARAPL2 Antibody (7895) <br> Blue: DAPI staining
Western blot analysis of GABARAPL2 in human brain tissue lysate with GABARAPL2 antibody at (A) 1 and (B) 2 μg/ml.
Trial Size

GABARAPL2 Antibody

CATALOG NUMBER: 7895

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (100%)
Immunogen:
GABARAPL2 antibody was raised against a 15 amino acid peptide near the carboxy terminus of human GABARAPL2.
The immunogen is located within the last 50 amino acids of GABARAPL2.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
GABARAPL2 antibody can be used for detection of GABARAPL2 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry at 5 μg/mL. For Immunoflorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse and rat samples and Immunofluorescence in mouse and rat samples. All other applications and species not yet tested.
Specificity:
GABARAPL2 antibody is human, mouse and rat reactive. Multiple isoforms of GABARAPL2 are known to exist.
Positive Control 1:
Cat. No. 1303 - Human Brain Tissue Lysate
Predicted Molecular Weight:
Predicted: 13 kDa
Observed: 13 kDa
Purification:
GABARAPL2 antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
GABARAPL2 antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
GABARAPL2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Ncbi Official Symbol:
GABARAPL2
Additional Names:
GABARAPL2 Antibody: ATG8, GEF2, ATG8C, GEF-2, GATE16, GATE-16, FLC3A, Gamma-aminobutyric acid receptor-associated protein-like 2, Ganglioside expression factor 2
Protein Accession Number:
NP_009216
Protein Gi Number:
6005768
Ncbi Gene Id Number:
11345
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Gamma-aminobutyric acid (GABA) is the main inhibitory transmitter by increasing a Cl-conductance that inhibits neuronal firing in the central nervous system (1). It has been shown to activate both ionotropic (GABAA) and metabotropic (GABAB) receptors as well as a third class of receptors called GABAC (2). GABARAPL2 (GABAA receptor-associated protein-like 2), also known as GATE16, was initially identified as a membrane transport modulator and is a mammalian ortholog to the autophagy protein ATG8 (3,4). It is thought that GABARAPL2 and other members of the ATG8 family act as scaffolds for assembly of the Unc-51 like kinase (ULK) complex in the formation of autophagosomes (5).
Background Reference 1:
Cherubini E, Gaiarsa JL, and Ben-Ari Y. GABA: an excitatory transmitter in early postnatal life. Trends Neurosci.1991; 14:515-19.
Background Reference 2:
Dirkx R Jr, Thomas A, Li L, et al. Targeting of the 67 kDa isoform of glutamic acid decarboxylase to intracellular organelles is mediated by its interaction with the NH2-terminal region of the 65 kDa isoform of glutamic acid decarboxylase. J. Biol. Chem. 1995; 270:2241-6.
Background Reference 3:
Sagiv Y, Legesse-Miller A, Porat A, et al. GATE-16, a membrane transport modulator, interacts with NSF and the Golgi v-SNARE GOS-28. EMBO J. 2000; 19:1494-504.
Background Reference 4:
Paz Y, Elazar Z, and Fass D. Structure of GATE-16, membrane transport modulator and mammalian ortholog of autophagocytosis factor Aut7p. J. Biol. Chem. 2000; 275:25445-50.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

7895

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

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$35 Dry Ice Shipment fee may be required on some items.

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