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45-646 (0.3ug/ml) staining of HeLa cell lysate (A) + peptide (B) (35ug protein in RIPA buffer). Detected by chemiluminescence.
45-646 (3.75ug/ml) staining of paraffin embedded Human Tonsil. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
45-646 (3.75ug/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
45-646 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear/nuclear speckle staining. Actin filaments
45-646 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear staining. Actin filaments were stained wit
45-646 Flow cytometric analysis of paraformaldehyde fixed HEK293 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) f
45-646 (0.3ug/ml) staining of HeLa cell lysate (A) + peptide (B) (35ug protein in RIPA buffer). Detected by chemiluminescence.

GABPB2 Antibody

CATALOG NUMBER: 45-646

Clonality:
Polyclonal
Tested Applications:
ELISA, Flow, IF, IHC, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Dog
Immunogen:
The immunogen for this antibody is: C-SSENSSKATDETG
Conjugate:
Unconjugated
Tested Applications:
ELISA, Flow, IF, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 45+38kDa bands observed in lysates of cell line HeLa (calculated MW of 42.5kDa according to NP_005245.2, NP_001307844.1 and of 38.1kDa according to NP_002032.2). An additional band of unknown identity was also consistently observed at 22kDa and was successfully blocked by incubation with the immunising peptide. Recommended concentration: 0.3-1ug/ml. Primary incubation 1 hour at room temperature.Immunohistochemistry:Paraffin embedded Human Tonsil and Spleen. Recommended concentration: 3.75ug/ml.Immunofluorescence: Strong expression of the protein seen in the nuclei of HeLa and U2OS cells. Recommended concentration: 10ug/ml.

Flow Cytometry: Flow cytometric analysis of HEK293 cells. Recommended concentration: 10ug/ml.

Positive Control 1:
Cat. No. 1201 - HeLa Cell Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
GABPB2
Additional Names:
GABPB2, GA binding protein transcription factor, beta subunit 2, BABPB2, E4TF1, E4TF1-47, E4TF1-53, E4TF1B, GABPB, GABPB1, NRF2B1, NRF2B2, GA binding protein transcription factor, beta subunit 1, 53kDa, GA binding protein transcription factor, beta subunit 2, 47kDa, nuclear respiratory factor-2 subunit beta
Protein Accession Number:
NP_005245.2,NP_002032.2,NP_001307844.1
Protein Gi Number:
8051593
Ncbi Gene Id Number:
2553
Background Reference 1:
O'Leary DA, Koleski D, Kola I, Hertzog PJ, Ristevski S. Identification and expression analysis of alternative transcripts of the mouse GA-binding protein (Gabp) subunits alpha and beta1. Gene. 2005 Jan 3;344:79-92. Epub 2004 Nov 23.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-646

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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