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Western blot analysis of GABRA4 Antibody in CEM cell line lysates (35ug/lane)
GABRA4 Antibody IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of GABRA4 Antibody in CEM cell line lysates (35ug/lane)

GABRA4 Antibody

CATALOG NUMBER: 63-880

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This GABRA4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 350-377 amino acids from the Central region of human GABRA4.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
62 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
GABRA4
Additional Names:
Gamma-aminobutyric acid receptor subunit alpha-4, GABA(A) receptor subunit alpha-4, GABRA4
Protein Accession Number:
P48169
Protein Gi Number:
206729865
Ncbi Gene Id Number:
2557
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
GABRA4 is the major inhibitory neurotransmitter in the mammalian brain where it acts at GABA-A receptors, which are ligand-gated chloride channels. Chloride conductance of these channels can be modulated by agents such as benzodiazepines that bind to the GABA-A receptor.
Background Reference 1:
Guilmatre,A., et.al., Arch. Gen. Psychiatry 66 (9), 947-956 (2009)
Background Reference 2:
Maldonado-Aviles,J.G., et.al., AmPsychiatry 166 (4), 450-459 (2009)
Background Reference 3:
Agrawal,A., et.al., Addiction 104 (3), 471-477 (2009)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

63-880

List Size:
400 ul

List Price:

$495.00

Datasheet
Shipping Info

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