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Western blot analysis of extracts of various cell lines, using GABRA6 antibody (14-612) at 1:3000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 10s.
Western blot analysis of extracts of various cell lines, using GABRA6 antibody (14-612) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 10s.

GABRA6 Antibody

CATALOG NUMBER: 14-612

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 344-453 of human GABRA6 (NP_000802.2).
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
WB: 1:500 - 1:2000
Positive Control 1:
SH-SY5Y
Positive Control 2:
HT-1080
Positive Control 3:
Mouse brain
Positive Control 4:
Mouse liver
Positive Control 5:
Rat brain
Predicted Molecular Weight:
Observed: 51kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
GABRA6
Additional Names:
Gabra6, gamma-aminobutyric acid (GABA) A receptor, subunit alpha 6, RP23-288I22.1, Gabra-6, alpha6, GABA(A) receptor subunit alpha-6, OTTMUSP00000006689, gamma-aminobutyric acid (GABA-A) receptor, subunit alpha 6, gamma-aminobutyric acid A recptor, subuni
Ncbi Gene Id Number:
2559
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
GABA is the major inhibitory neurotransmitter in the mammalian brain where it acts at GABA-A receptors, which are ligand-gated chloride channels. Chloride conductance of these channels can be modulated by agents such as benzodiazepines that bind to the GABA-A receptor. At least 16 distinct subunits of GABA-A receptors have been identified.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

14-612

List Size:
100 uL

List Price:

$419.00

Out of stock

Datasheet
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