Start Your Project

🛍️Explore our Promotions page to discover current deals, limited-time offers, and more!🌟

Western blot analysis of lysate from rat brain tissue lysate, using GAD2 Antibody at 1:1000.
Western blot analysis in Jurkat cell line lysates (35ug/lane).
GAD2 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Confocal immunofluorescent analysis of GAD2 Antibody with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Western blot analysis of lysate from rat brain tissue lysate, using GAD2 Antibody at 1:1000.

GAD2 Antibody

CATALOG NUMBER: 55-644

Clonality:
Polyclonal
Tested Applications:
IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse, Pig, Rat
Immunogen:
This GAD2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 109-138 amino acids from the Central region of human GAD2.
Conjugate:
Unconjugated
Tested Applications:
IF, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For IF starting dilution is: 1:10~50
Predicted Molecular Weight:
65 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
GAD2
Additional Names:
Glutamate decarboxylase 2, 65 kDa glutamic acid decarboxylase, GAD-65, Glutamate decarboxylase 65 kDa isoform, GAD2, GAD65
Protein Accession Number:
Q05329
Protein Gi Number:
1352216
Ncbi Gene Id Number:
2572
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes one of several forms of glutamic aciddecarboxylase, identified as a major autoantigen ininsulin-dependent diabetes. The enzyme encoded is responsible forcatalyzing the production of gamma-aminobutyric acid fromL-glutamic acid. A pathogenic role for this enzyme has beenidentified in the human pancreas since it has been identified as anautoantibody and an autoreactive T cell target in insulin-dependentdiabetes. This gene may also play a role in the stiff man syndrome.Alternative splicing results in multiple transcript variants thatencode the same protein.
Background Reference 1:
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Background Reference 2:
Jia, P., et al. Schizophr. Res. 122 (1-3), 38-42 (2010) :
Background Reference 3:
Ruano, G., et al. Pharmacogenomics 11(7):959-971(2010)
Background Reference 4:
Pinheiro, A.P., et al. Am. J. Med. Genet.Neuropsychiatr. Genet. 153B (5), 1070-1080 (2010) :

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

55-644

List Size:
400 ul

List Price:

$495.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

Customize your order

Bulk Pricing Available

Custom Formulations: azide free, endotoxin testing & endotoxin removal.

Pair your antibody with a Lysate, Peptide, Control, & /OR Secondary Antibody.

Contact our Customer Care Team for more information

Custom Antibody Services

Speak to one of our custom antibody specialists to discuss your next custom antibody project.

New & Featured Products

HIV-1 p24 Antibody [9D2F6] (HRP)
HIV-1 p24 Antibody [9D2F6] (HRP)

CATALOG NUMBER: PM-7369-HRP

HIV-1 p24 Antibody [9D2F6] (biotin)
HIV-1 p24 Antibody [9D2F6] (biotin)

CATALOG NUMBER: PM-7369-biotin

HIV-1 p24 Antibody [9D2F6]
HIV-1 p24 Antibody [9D2F6]

CATALOG NUMBER: PM-7369