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45-648 (0.5ug/ml) staining of Human Brain (Hippocampus) lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
45-648 (2.5ug/ml) staining of paraffin embedded Human Colon. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. <strong>This data is from a previous batch, not on sale.</strong>
45-648 (2.5ug/ml) staining of paraffin embedded Human Cerebellum. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. <strong>This data is from a previous batch, not on sale.</strong>
45-648 (0.5ug/ml) staining of Human Brain (Hippocampus) lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

GAD65 Antibody

CATALOG NUMBER: 45-648

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog
Immunogen:
The immunogen for this antibody is: C-TLEDNEERMSRLSK
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 65kDa band observed in Human Brain (Hippocampus) lysates (calculated MW of 65.4kDa according to NP_000809.1). Recommended concentration: 0.5-1.5ug/ml. Primary incubation was 1 hour.
Specificity:
This antibody is expected to recognise both reported variants.
Positive Control 1:
Cat. No. 1303 - Human Brain Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
GAD2
Additional Names:
GAD2, GAD65, glutamate decarboxylase 2 (pancreatic islets and brain, 65kDa), Glutamate decarboxylase-2 (pancreas), glutamate decarboxylase 2 (pancreatic islets and brain, 65kD), RP11-420F12.2, MGC161605, MGC161607, Glutamate decarboxylase-2 (pancreas), glutamate decarboxylase 2
Protein Accession Number:
NP_000809.1,NP_001127838.1
Protein Gi Number:
4503875
Ncbi Gene Id Number:
2572
Background Reference 1:
Meyre D, Boutin P, Tounian A, Deweirder M, Aout M, Jouret B, Heude B, Weill J, Tauber M, Tounian P, Froguel P. Is Glutamate Decarboxylase 2 (GAD2) a genetic link between low birth weight and subsequent development of obesity in children? J Clin Endocrinol Metab. 2005 Jan 25;

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-648

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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