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GADD45 gamma Antibody

CATALOG NUMBER: 46-662

Clonality:
Polyclonal
Tested Applications:
ELISA
Host Species:
Goat
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Homology:
Expected Species Reactivity based on sequence homology: Human, Mouse, Rat
Immunogen:
The immunogen for this antibody is: C-RMQGAGKALHE
Conjugate:
Unconjugated
Tested Applications:
ELISA
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Preliminary experiments gave an approx 23kDa band in Human Testis and Thyroid Gland lysates and in lysates of cell line A549 after 0.3ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 17.1kDa according to NP_006696.1. The 23kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
GADD45G
Additional Names:
growth arrest and DNA-damage-inducible, gamma, CR6, DDIT2, GADD45gamma, GRP17, GADD45-gamma, gadd-related protein, 17 kD, growth arrest and DNA-damage-inducible gamma
Protein Accession Number:
NP_006696.1
Protein Gi Number:
5729836
Ncbi Gene Id Number:
10912
Background Reference 1:
Zerbini LF, Wang Y, Czibere A, Correa RG, Cho JY, Ijiri K, Wei W, Joseph M, Gu X, Grall F, Goldring MB, Zhou JR, Libermann TA. NF-kappa B-mediated repression of growth arrest- and DNA-damage-inducibleproteins 45alpha and gamma is essential for cancer cell survival. Proc Natl Acad Sci USA. 2004 Sep 14;101(37):13618-23.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

46-662

List Size:
0.1 mg

List Price:

$468.00

Datasheet
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