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Western blot analysis of extracts of Rat brain, using GADD45A antibody (16-952) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 3min.
Immunohistochemistry of paraffin-embedded human colon using GADD45A antibody (16-952) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human lung cancer using GADD45A antibody (16-952) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using GADD45A antibody (16-952) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using GADD45A antibody (16-952) at dilution of 1:200 (40x lens).
Immunofluorescence analysis of U-2 OS cells using GADD45A antibody (16-952) at dilution of 1:100. Blue: DAPI for nuclear staining.
Western blot analysis of extracts of Rat brain, using GADD45A antibody (16-952) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 3min.

GADD45A Antibody

CATALOG NUMBER: 16-952

Clonality:
Polyclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
A synthetic peptide of Human GADD45A.
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:50 - 1:200
Positive Control 1:
Rat brain
Predicted Molecular Weight:
Observed: 18kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
GADD45A
Additional Names:
Growth arrest and DNA damage-inducible protein GADD45 alpha, DNA damage-inducible transcript 1 protein, DDIT-1, GADD45A, DDIT1, GADD45
Ncbi Gene Id Number:
1647
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene is a member of a group of genes whose transcript levels are increased following stressful growth arrest conditions and treatment with DNA-damaging agents. The protein encoded by this gene responds to environmental stresses by mediating activation of the p38/JNK pathway via MTK1/MEKK4 kinase. The DNA damage-induced transcription of this gene is mediated by both p53-dependent and -independent mechanisms. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.[provided by RefSeq, Dec 2010]

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

16-952

List Size:
50 uL

List Price:

$309.00

Out of stock

Datasheet
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