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Western blot analysis of GADD45A Antibody in Y79 cell line lysates (35ug/lane)
Flow cytometric analysis of NCI-H460 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of GADD45A using rabbit polyclonal GADD45A Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the GADD45A gene.
Western blot analysis of GADD45A Antibody in Y79 cell line lysates (35ug/lane)

GADD45A Antibody

CATALOG NUMBER: 61-503

Clonality:
Polyclonal
Tested Applications:
Flow, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse, Rat, Hamster, Bovine
Immunogen:
This GADD45A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 133-165 amino acids from the C-terminal region of human GADD45A.
Conjugate:
Unconjugated
Tested Applications:
Flow, WB
Application Note:
For WB starting dilution is: 1:1000
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
18 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
GADD45A
Additional Names:
Growth arrest and DNA damage-inducible protein GADD45 alpha, DNA damage-inducible transcript 1 protein, DDIT-1, GADD45A, DDIT1, GADD45
Protein Accession Number:
P24522
Protein Gi Number:
120751
Ncbi Gene Id Number:
1647
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
GADD45A responds to environmental stresses by mediating activation of the p38/JNK pathway via MTK1/MEKK4 kinase. The GADD45A gene is a member of a group of genes whose transcript levels are increased following stressful growth arrest conditions and treatment with DNA-damaging agents. The DNA damage-induced transcription of this gene is mediated by both p53-dependent and -independent mechanisms.
Background Reference 1:
Li,L.S., J. Biol. Chem. 283 (31), 21394-21403 (2008)
Background Reference 2:
Al-Romaih,K., Neoplasia 10 (5), 471-480 (2008)
Background Reference 3:
Zhu,Q.S., Cancer Res. 68 (8), 2895-2903 (2008)
Background Reference 4:
Kearsey,J.M., Oncogene 11 (9), 1675-1683 (1995)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

61-503

List Size:
400 ul

List Price:

$495.00

Datasheet
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