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To detect hGalectin-1 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Biotinylated Anti-Human Galectin-1 (38-141) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hGalectin-1.
To detect hGalectin-1 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hGalectin-1 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
To detect hGalectin-1 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hGalectin-1 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
To detect hGalectin-1 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Biotinylated Anti-Human Galectin-1 (38-141) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hGalectin-1.

Galectin-1 Antibody

CATALOG NUMBER: 38-140

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hGalectin-1. Human Galectin-1 specific antibody was purified by affinity chromatography employing immobilized hGalectin-1 matrix.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
ELISA:
Indirect:
To detect hGalectin-1 by indirect ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hGalectin-1.
Sandwich
To detect hGalectin-1 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our biotinylated Anti-Human Galectin-1as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hGalectin-1.
Western Blot:
To detect hGalectin-1 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hGalectin-1 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Clonality:
Polyclonal
physical-state:
Lyophilized
Concentration:
batch dependent
Storage Conditions:
Galectin-1 antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
Ncbi Official Symbol:
LGALS1
Additional Names:
GBP, GAL1Galectin-1, 14 kDa laminin-binding protein, Gal-1
Protein Accession Number:
P09382
Protein Gi Number:
126155
Ncbi Gene Id Number:
3956
User Note:
Centrifuge vial prior to opening.
Background:
The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. Galectin 1 may act as an autocrine negative growth factor that regulates cell proliferation. It also may regulate cell apoptosis and cell differentiation. It binds beta-galactoside, CD45, CD3 and CD4. Galectin 1 inhibits CD45 protein phosphatase activity and therefore the dephosphorylation of Lyn kinase. It is found in cardiac, smooth, and skeletal muscle, neurons, thymus, kidney, placenta and hematopoietic cells.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

38-140

List Size:
0.1 mg

List Price:

$408.00

Datasheet
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