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IHC testing of FFPE human placenta with LGALS13 antibody (clone PP13/1165). Required HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH 9 or 10mM Citrate buffer, pH 6, for 10-20.
SDS-PAGE Analysis of Purified, BSA-Free LGALS13 Antibody (clone PP13/1165). Confirmation of Integrity and Purity of the Antibody.
IHC testing of FFPE human placenta with LGALS13 antibody (clone PP13/1165). Required HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH 9 or 10mM Citrate buffer, pH 6, for 10-20.

Galectin 13 Antibody [PP13/1165]

CATALOG NUMBER: 33-702

Clonality:
Monoclonal
Tested Applications:
ELISA, Flow, IF, IHC-P, WB
Host Species:
Mouse
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
Human
Immunogen:
A human partial recombinant protein corresponding to amino acids 23-134 was used as the immunogen for the LGALS13 antibody.
Conjugate:
Unconjugated
Tested Applications:
ELISA, Flow, IF, IHC-P, WB
Application Note:
IF: 1-2 ug/ml
Flow Cytometry: 0.5-1 ug/million cells in 0.1ml
ELISA: order the BSA-free formulation for coating
IHC (FFPE): 0.5-1 ug/ml for 30 min at RT
Titering of the LGALS13 antibody may be required for optimal performance.
Purification:
Protein G affinity chromatography
Clonality:
Monoclonal
Isotype:
IgG2b, kappa
Physical State:
Liquid
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Storage Conditions:
Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
Ncbi Official Symbol:
LGALS13
Ncbi Gene Id Number:
29124
User Note:
Optimal dilutions for each application to be determined by the researcher
Background:
It recognizes a 32kDa protein, which is identified as homodimer of LGALS13/Galectin 13 (also known as PP13). Galectins are a family of soluble beta-galactoside-binding lectins that modulate cell-to-cell adhesion and cell-to-extracellular matrix (ECM) interactions and play a role in tumor progression, pre-mRNA splicing and apoptosis. One such member, LGALS13, is a 139 amino acid protein that contains one galectin domain. Possessing lysophospholipase activity, LGALS13 exists as a disulfide-linked homodimer. LGALS13 is suggested to have a developmental role in the placenta and may display immunobiological activity in feto-maternal blood-spaces. Screening for LGALS13/Galectin 13 in maternal serum during the first trimester of pregnancy may serve as a diagnostic marker to predict preterm pre-eclampsia.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

33-702

List Size:
100 ug

List Price:

$626.00

Datasheet
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