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43-637 (0.3ug/ml) staining of Human (A) and Mouse (B) Colon lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
43-637 (0.3ug/ml) staining of Human (A) and Mouse (B) Colon lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Galectin 3 Antibody

CATALOG NUMBER: 43-637

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human, Mouse
Homology:
Expected Species Reactivity based on sequence homology: Rat
Immunogen:
The immunogen for this antibody is: CNTKLDNNWGREERQ
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 30kDa band observed in Human and Mouse Colon lysates (calculated MW of 26.2kDa according to NP_002297.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Lotz et al, Proc Natl Acad Sci U S A. 1993 Apr 15;90(8):3466-70. PMID: 7682704). Recommended concentration: 0.3-1ug/ml.
Predicted Molecular Weight:
Approx 30kDa
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
Galectin 3
Additional Names:
LGALS3, lectin, galactoside-binding, soluble, 3 , CBP35, GAL3, GALBP, GALIG, LGALS2, MAC2 , IgE-binding protein, MAC-2 antigen, carbohydrate-binding protein 35, galactose-specific lectin 3, galectin 3, galectin-3 internal gene, laminin-binding protein, lectin, galactoside-binding, soluble, 3 (galectin 3)
Protein Accession Number:
NP_002297.2
Protein Gi Number:
215274262
Ncbi Gene Id Number:
3958
Background Reference 1:
Pilette C, Colinet B, Kiss R, Andre S, Kaltner H, Gabius HJ, Delos M, Vaerman JP, Decramer M, Sibille Y. Increased galectin-3 expression and intraepithelial neutrophils in small airways in severe chronic obstructive pulmonary disease.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

43-637

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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