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Western blot analysis of various lysates using Galectin 3/LGALS3 Rabbit mAb at 1:9000 dilution incubated overnight at 4°C. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 25 μg per lane. | Blocking buffer: 3 % nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Exposure time: 10s.
Immunofluorescence analysis of HeLa cells using Galectin 3/LGALS3 Rabbit mAb at a dilution of 1:400 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunoprecipitation of Galectin 3/LGALS3 from 300 µg extracts of Hela cells was performed using 3 µg of Galectin 3/LGALS3 Rabbit mAb. Rabbit IgG isotype control was used to precipitate the Control IgG sample. IP samples were eluted with 1X reducing Laemmli Buffer. The Input lane represents 10% of the total input. Western blot analysis of immunoprecipitates was conducted using Galectin 3/LGALS3 Rabbit mAb at 1:10000 dilution.
Flow cytometry: 1X10^6 Jurkat cells (negative control,left) and MCF7 cells (right) were surface-stained with Galectin 3/LGALS3 Rabbit mAb or Rabbit IgG isotype control, followed by FITC conjugated goat anti-Rabbit pAb staining. Non-fluorescently stained cells were used as blank control (red line).
Flow cytometry: 1X10^6 MCF7 cells were surface-stained with Rabbit IgG isotype control or Galectin 3/LGALS3 Rabbit mAb, followed by FITC conjugated goat anti-Rabbit pAb staining.
Western blot analysis of various lysates using Galectin 3/LGALS3 Rabbit mAb at 1:9000 dilution incubated overnight at 4°C. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 25 μg per lane. | Blocking buffer: 3 % nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Exposure time: 10s.

Galectin 3/LGALS3 Rabbit Recombinant Antibody (83-112)

CATALOG NUMBER: 83-112

Clonality:
Monoclonal
Tested Applications:
ELISA, FC, IF/ICC, IP, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Conjugate:
Unconjugated
Tested Applications:
ELISA, FC, IF/ICC, IP, WB
Application Note:
WB,1:9000 - 1:54000, IF/ICC,1:200-1:800, IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells, FC,1:100 - 1:500, ELISA,Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Positive Control 1:
HeLa,MCF7,U-87 MG
Predicted Molecular Weight:
26kDa
purification:
Affinity purification
Clonality:
Monoclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3.
Concentration:
Batch dependent
Storage Conditions:
Store at -20℃. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
LGALS3
Additional Names:
L31; GAL3; MAC2; CBP35; GALBP; GALIG; LGALS2
Protein Accession Number:
P17931
Ncbi Gene Id Number:
3958
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a member of the galectin family of carbohydrate binding proteins. Members of this protein family have an affinity for beta-galactosides. The encoded protein is characterized by an N-terminal proline-rich tandem repeat domain and a single C-terminal carbohydrate recognition domain. This protein can self-associate through the N-terminal domain allowing it to bind to multivalent saccharide ligands. This protein localizes to the extracellular matrix, the cytoplasm and the nucleus. This protein plays a role in numerous cellular functions including apoptosis, innate immunity, cell adhesion and T-cell regulation. The protein exhibits antimicrobial activity against bacteria and fungi. Alternate splicing results in multiple transcript variants.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

83-112

List Size:
100 uL

List Price:

$540.00

Datasheet
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