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Western blot analysis of GALNT10 in rat brain tissue lysate with GALNT10 antibody at (A) 1 and (B) 2 μg/mL.
Immunohistochemistry of GALNT10 in human brain tissue with GALNT10 antibody at 2.5 μg/mL.
Immunofluorescence of GALNT10 in human brain tissue with GALNT10 antibody at 20 μg/mL.
Western blot analysis of GALNT10 in rat brain tissue lysate with GALNT10 antibody at (A) 1 and (B) 2 μg/mL.
Trial Size

GALNT10 Antibody

CATALOG NUMBER: 5281

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
GALNT10 antibody was raised against a 16 amino acid synthetic peptide near the amino terminus of human GALNT10.
The immunogen is located within amino acids 80 - 130 of GALNT10.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
GALNT10 antibody can be used for detection of GALNT10 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in rat samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1463 - Rat Brain Tissue Lysate
Positive Control 2:
Cat. No. 10-301 - Human Brain Tissue Slide
Purification:
GALNT10 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
GALNT10 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
GALNT10 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
GALNT10
Additional Names:
GALNT10 Antibody: GALNACT10, PPGALNACT10, PPGANTASE10, Polypeptide N-acetylgalactosaminyltransferase 10, Polypeptide GalNAc transferase 10, GalNAc-T10
Protein Accession Number:
NP_938080
Protein Gi Number:
38195091
Ncbi Gene Id Number:
55568
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
GALNT10 Antibody: Protein glycosylation is an important biological process that is carried out by a large family of glycosyltransferases that catalyze the synthesis of oligosaccharides and glycoconjugates. Polypeptide GalNAc transferases initiate the synthesis of mucin-type oligosaccharides by transferring GalNAc from UDP-GalNAc to the hydroxyl group of either a serine or threonine residue on the polypeptide acceptor. Polypeptide galactoaminyltransferase 10 (GALNT10) belongs to the polypeptide N-acetylgalactosaminyl-transferase (pp-GalNAc-T) protein family. Following expression in insect cells, recombinant GALNT10 showed significant GalNAcT activity toward mucin-derived peptides, and it utilized both non-glycosylated and glycosylated peptide substrates. GALNT10 mRNA is highly expressed in several distinct hypothalamic, thalamic, and amygdaloid nuclei in mouse brain. At least four isoforms of GALNT10 are known to exist.
Background Reference 1:
Amado M, Almeida R, Schwientek T, et al. Identification and characterization of large galactosyltransferase gene families: galactosyltransferases for all functions. Biochim. Biophys. Acta1999; 1473:35-53.
Background Reference 2:
Nelson PA, Sutcliffe JG, and Thomas EA. A new UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase mRNA exhibits predominant expression in the hypothalamus, thalamus and amygdala for mouse forebrain. Gene Express. Patterns2002; 1:95-9.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

5281

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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