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Western blot analysis in Hela cell line lysates (35ug/lane).
Flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis in Hela cell line lysates (35ug/lane).

GALNT4 Antibody

CATALOG NUMBER: 55-079

Clonality:
Polyclonal
Tested Applications:
Flow, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse
Immunogen:
This GALNT4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 93-120 amino acids from the N-terminal region of human GALNT4.
Conjugate:
Unconjugated
Tested Applications:
Flow, WB
Application Note:
For WB starting dilution is: 1:1000
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
67 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
GALNT4
Additional Names:
Polypeptide N-acetylgalactosaminyltransferase 4, Polypeptide GalNAc transferase 4, GalNAc-T4, pp-GaNTase 4, Protein-UDP acetylgalactosaminyltransferase 4, UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 4, GALNT4
Protein Accession Number:
Q8N4A0
Protein Gi Number:
338817878
Ncbi Gene Id Number:
1.00528E+12
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a member of theUDP-N-acetyl-alpha-D-galactosamine:polypeptideN-acetylgalactosaminyltransferase (GalNAc-T) family of enzymes.GalNAc-Ts initiate mucin-type O-linked glycosylation in the Golgiapparatus by catalyzing the transfer of GalNAc to serine andthreonine residues on target proteins. They are characterized by anN-terminal transmembrane domain, a stem region, a lumenal catalyticdomain containing a GT1 motif and Gal/GalNAc transferase motif, anda C-terminal ricin/lectin-like domain. GalNAc-Ts have different,but overlapping, substrate specificities and patterns ofexpression. In vitro, the encoded protein can complement otherGalNAc-Ts in the complete O-glycosylation of the mucin-1 tandemrepeat and can O-glycosylate the P-selectin glycoprotein ligand-1molecule. The coding region of this gene is contained within asingle exon.
Background Reference 1:
O'Halloran, A.M., et al. J. Thromb. Thrombolysis 27(2):175-184(2009)
Background Reference 2:
Argueso, P., et al. Invest. Ophthalmol. Vis. Sci. 44(1):86-92(2003)
Background Reference 3:
Hassan, H., et al. J. Biol. Chem. 275(49):38197-38205(2000)
Background Reference 4:
Bennett, E.P., et al. J. Biol. Chem. 273(46):30472-30481(1998)

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

55-079

List Size:
400 ul

List Price:

$495.00

Datasheet
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