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Western blot analysis of lysates from A431, K562 cell line (from left to right), using GALNT5 Antibody at 1:1000 at each lane.
GALNT5 Antibody IHC analysis in formalin fixed and paraffin embedded human normal lung tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of lysates from A431, K562 cell line (from left to right), using GALNT5 Antibody at 1:1000 at each lane.

GALNT5 Antibody

CATALOG NUMBER: 64-219

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This GALNT5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 24-53 amino acids from the N-terminal region of human GALNT5.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
106 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
GALNT5
Additional Names:
Polypeptide N-acetylgalactosaminyltransferase 5, Polypeptide GalNAc transferase 5, GalNAc-T5, pp-GaNTase 5, Protein-UDP acetylgalactosaminyltransferase 5, UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 5, GALNT5
Protein Accession Number:
Q7Z7M9
Protein Gi Number:
51315940
Ncbi Gene Id Number:
11227
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
GALNT5 can catalyze the initial reaction in O-linked oligosaccharide biosynthesis,the transfer of an N-acetyl-D-galactosamine residue to a serine or threonine residue on the protein receptor. GALNT5 has activity toward EA2 peptide substrate, but it has a weak activity toward Muc2 or Muc1b substrates.
Background Reference 1:
Simmons, A.D., et al. Hum. Mol. Genet. 8(12):2155-2164(1999)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

64-219

List Size:
400 ul

List Price:

$495.00

Datasheet
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