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Western Blot at 1:1000 dilution Lane 1: CCRF-CEM whole cell lysate Lane 2: K562 whole cell lysate Lysates/proteins at 20 ug per lane.
Flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western Blot at 1:1000 dilution Lane 1: CCRF-CEM whole cell lysate Lane 2: K562 whole cell lysate Lysates/proteins at 20 ug per lane.

GALR1 Antibody

CATALOG NUMBER: 63-904

Clonality:
Polyclonal
Tested Applications:
Flow, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This GALR1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 174-200 amino acids from the Central region of human GALR1.
Conjugate:
Unconjugated
Tested Applications:
Flow, WB
Application Note:
For WB starting dilution is: 1:1000
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
39 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
GALR1
Additional Names:
Galanin receptor type 1, GAL1-R, GALR-1, GALR1, GALNR, GALNR1
Protein Accession Number:
P47211
Protein Gi Number:
311033447
Ncbi Gene Id Number:
2587
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
GALR1 elicits a range of biological effects by interaction with specific G-protein-coupled receptors. Galanin receptors are seven-transmembrane proteins shown to activate a variety of intracellular second-messenger pathways. GALR1 inhibits adenylyl cyclase via a G protein of the Gi/Go family. GALR1 is widely expressed in the brain and spinal cord, as well as in peripheral sites such as the small intestine and heart.
Background Reference 1:
Borroto-Escuela,D.O. Biochem. Biophys. Res. Commun. 393 (4), 767-772 (2010)
Background Reference 2:
Gratacos,M. Am. J. Med. Genet.Neuropsychiatr. Genet. 150B (6), 808-816
Background Reference 3:
Marroni,F. Circ Cardiovasc Genet(4), 322-328 (2009)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

63-904

List Size:
400 ul

List Price:

$495.00

Datasheet
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