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Western blot testing of human 1) HT20, 2) HEK293, 3) A431, 4) MCF7, 5) HEPG2 and 6) A549 cell lysate with Plakoglobin antibody (clone 15F11). Predicted molecular weight: 80-87 kDa.
Western blot testing of human 1) HT20, 2) HEK293, 3) A431, 4) MCF7, 5) HEPG2 and 6) A549 cell lysate with Plakoglobin antibody (clone 15F11). Predicted molecular weight: 80-87 kDa.

Gamma Catenin Antibody [15F11]

CATALOG NUMBER: 33-586

Clonality:
Monoclonal
Tested Applications:
IF, IP, WB
Host Species:
Mouse
Species Reactivity:
Bovine, Cat, Chicken, Dog, Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
Bovine, Cat, Chicken, Dog, Human, Mouse, Rat
Immunogen:
Recombinant full length chicken gamma Catenin was used as the immunogen for the Plakoglobin antibody.
Conjugate:
Unconjugated
Tested Applications:
IF, IP, WB
Application Note:
Immunofluorescence: 1-2 ug/ml
WB: 0.5-1 ug/ml
Immunoprecipitation: suitable
The stated application concentrations are suggested starting amounts. Titration of the Plakoglobin antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Purification:
Protein G affinity chromatography
Clonality:
Monoclonal
Isotype:
IgG1, kappa
physical-state:
Liquid
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Storage Conditions:
Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
Ncbi Official Symbol:
JUP
Additional Names:
Junction plakoglobin, Catenin gamma, Desmoplakin III, Desmoplakin-3, JUP, CTNNG, DP3
Ncbi Gene Id Number:
3728
User Note:
Optimal dilutions for each application to be determined by the researcher
Background:
Plakoglobin, or gamma Catenin, is a common junctional plaque protein. The membrane-associated plaques are architectural elements in an important strategic position to influence the arrangement and function of both the cytoskeleton and the cells within the tissue. The presence of Plakoglobin in both the desmosomes and in the intermediate junctions suggests that it plays a central role in the structure and function of submembranous plaques. Acts as a substrate for VE-PTP and is required by it to stimulate VE-cadherin function in endothelial cells. Can replace beta-catenin in E-cadherin/catenin adhesion complexes which are proposed to couple cadherins to the actin cytoskeleton (By similarity). [UniProt]

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

33-586

List Size:
100 ug

List Price:

$626.00

Datasheet
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