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Western blot analysis of extracts of various cell lines, using GANAB antibody (15-233) at 1:3000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 10s.
Immunohistochemistry of paraffin-embedded rat brain using GANAB antibody (15-233) at dilution of 1:150 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using GANAB antibody (15-233) at dilution of 1:150 (40x lens).
Immunofluorescence analysis of C6 cells using GANAB antibody (15-233) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH-3T3 cells using GANAB antibody (15-233) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U-2 OS cells using GANAB antibody (15-233) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of C6 cells using GANAB antibody (15-233) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH-3T3 cells using GANAB antibody (15-233) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U-2 OS cells using GANAB antibody (15-233) at dilution of 1:100. Blue: DAPI for nuclear staining.
Western blot analysis of extracts of various cell lines, using GANAB antibody (15-233) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.

GANAB Antibody

CATALOG NUMBER: 15-233

Clonality:
Polyclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human GANAB (NP_001265123.1).
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:50 - 1:200
Positive Control 1:
U-87MG
Positive Control 2:
Raji
Positive Control 3:
HepG2
Positive Control 4:
HeLa
Positive Control 5:
Mouse brain
Positive Control 6:
Mouse liver
Predicted Molecular Weight:
Observed: 112kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
GANAB
Additional Names:
GANAB, G2AN, Glucosidase, alpha, Glucosidase, alpha neutral AB, Neutral alpha-glucosidase AB, Alpha-glucosidase 2, Glucosidase II subunit alpha, GLUII, KIAA0088, Neutral AB
Ncbi Gene Id Number:
23193
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes the alpha subunit of glucosidase II and a member of the glycosyl hydrolase 31 family of proteins. The heterodimeric enzyme glucosidase II plays a role in protein folding and quality control by cleaving glucose residues from immature glycoproteins in the endoplasmic reticulum. Expression of the encoded protein is elevated in lung tumor tissue and in response to UV irradiation. Mutations in this gene cause autosomal-dominant polycystic kidney and liver disease.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

15-233

List Size:
100 uL

List Price:

$436.00

Datasheet
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