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Western blot of rat cortex lysate showing phospho-specific immunolabeling of the ~50k GAP-43 protein phosphorylated at Ser41.
Western blot of rat cortex lysate showing phospho-specific immunolabeling of the ~50k GAP-43 protein phosphorylated at Ser41.

Gap-43 (phospho Ser41) Antibody

CATALOG NUMBER: XPS-1011

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
GAP-43 (Ser41) polyclonal antibody was raised against a synthetic phosphopeptide corresponding to amino acids residues surrounding the phospho-Ser41 of rat GAP-43.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
In some tissues the antibody also recognizes a higher molecular weight band that is also recognized by the Pan GAP-43 antibody and this band may be a GAP-43 aggregate or oligomer. Immunolabeling of the GAP-43 protein in Western blots of rat brain is blocked by the Ser41 phosphopeptide used as antigen but not by the corresponding non-phosphopeptide. Note: the GAP-43 protein migrates on SDS-PAGE as a 50 kDa band. The antibody is purified by sequential chromatography on phospho- and non-phosphopeptide affinity columns. Antibody dilutions and tissue load should be based on tissue type and expected phosphorylation state. Initial recommended range of dilutions: 1:500 to 1:2000. Applications include Dot blots (DB) and Western blots (WB). Immunohistochemistry (IHC) application has not yet been determined. Human, mouse and rat have 100% amino acid sequence identity with for the antigen used to raise the antibody. When internally tested under ideal conditions the working dilutions were 1:1000 for DB and WB.
Specificity:
Gap-43 antibody is specific for GAP-43 phosphorylated at Ser41.
Predicted Molecular Weight:
50
Purification:
Affinity Purified
Clonality:
Polyclonal
Physical State:
Liquid
Concentration:
batch dependent
Storage Conditions:
For long term storage -80°C is recommended, but shorter term storage at -20°C is also acceptable as aliquots may be taken without freeze/thawing due to the presence of 50% glycerol. Stock solutions are stable for a minimum of 1 year at -20°C.
Ncbi Official Symbol:
Gap43
Protein Accession Number:
P07936
Protein Gi Number:
128102
Ncbi Gene Id Number:
29423
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
GAP-43 is thought to have an important role in development and plasticity because it is expressed at high levels in neuronal growth cones during development and during axonal regeneration. There is also evidence from knockout animals that GAP-43 serves to amplify pathfinding signals from the growth cone. GAP-43 is thought to mediate at least some of these effects via interaction with actin. Importantly, phosphorylation at Ser41 by Protein Kinase C modulates the interaction of GAP-43 with actin.
Background Reference 1:
Hulo, S., Alberi, S., Laux, T., Muller, D., and Caroni, P., "A point mutant of GAP-43 induces enhanced short-term and long-term hippocampal plasticity," Eur. J. Neurosci., 15 (2002) 1976 - 1982.
Background Reference 2:
He, Q., Dent, E.W., and Meiri, K.F., "Modulation of actin filament behavior by GAP-43 (neuromodulin) is dependent on the phosphorylation status of serine 41, the protein kinase C site," J. Neurosci., 17 (1997) 3515 - 3524.
Background Reference 3:
Benowitz, L.I. and Routtenberg, A., "GAP-43: An intrinsic determinant of neuronal development and plasticit," Trends Neurosci., 20 (1997) 84 - 91.
Background Reference 4:
Strittmatter, S.M., Fankhauser, C., Huang, P.L., Mashimo, H., and Fishman, M.C., "Neuronal pathfinding is abnormal in mice lacking the neuronal growth cone protein GAP-43," Cell, 80 (1995) 445 - 452.

FOR RESEARCH USE ONLY

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Disclaimer:
This product is for research use only.

CATALOG NUMBER:

XPS-1011

List Size:
0.1 mL

List Price:

$540.00

Datasheet
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