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Western blot analysis of extract from JK, HL-60, HUVEC and HeLa cells using GAPDH Antibody.
Western blot analysis of extract from JK, HL-60, HUVEC and HeLa cells using GAPDH Antibody.

GAPDH Antibody

CATALOG NUMBER: 79-715

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
GAPDH antibody was raised against a peptide sequence around aa.252~256 (P-A-K-Y-D) derived from Human GAPDH.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
Western Blot: 1:500~1:1000
Specificity:
This antibody detects endogenous level of total GAPDH protein.
Predicted Molecular Weight:
37 kDa
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Storage Conditions:
Store antibody at -20°C for up to one year.
Ncbi Official Symbol:
GAPDH
Additional Names:
G3PD, GAPD, HEL-S-162eP, G3PD, MGC88685
Protein Accession Number:
NP_002037.2
Protein Gi Number:
7669492
Ncbi Gene Id Number:
2597
Background:
Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC By similarity. Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate.
Background Reference 1:
Ercolani L., Florence B., Denaro M., Alexander M. J. Biol. Chem. 263:15335-15341 (1988) Tisdale E. J. J. Biol. Chem. 277:3334-3341 (2002)

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

79-715

List Size:
0.1 mg

List Price:

$370.00

Datasheet
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