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Western blot analysis of GAPDH in 293, A431, A549, Daudi, HeLa, HepG2, Jurkat, K562, MOLT4, 3T3, Raji, Ramos, U937, human brain, mouse brain, rat brain, mouse lung, mouse liver, rat liver, and chicken small intestine lysate with Biotin-GAPDH antibody at 1 μg/mL.
Western blot analysis of GAPDH in 293, A431, A549, Daudi, HeLa, HepG2, Jurkat, K562, MOLT4, 3T3, Raji, Ramos, U937, human brain, mouse brain, rat brain, mouse lung, mouse liver, rat liver, and chicken small intestine lysate with Biotin-GAPDH antibody at 1 μg/mL.

GAPDH Antibody (biotin)

CATALOG NUMBER: 3781-biotin

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Chicken, Human, Mouse, Rabbit, Rat
Conjugate:
Biotin
Specifications
Host Species:
Rabbit
Species Reactivity:
Chicken, Human, Mouse, Rabbit, Rat
Immunogen:
Biotin-GAPDH antibody was raised against a synthetic peptide containing 16 amino acids near the carboxy terminus of GAPDH.
Conjugate:
Biotin
Tested Applications:
ELISA, WB
Application Note:
Biotin-GAPDH antibody can be used for detection of GAPDH by Western blot at 0.5 - 1 μ/ml.
Antibody validated: Western Blot in human, mouse, and rat samples. All other applications and species not yet tested.
Specificity:
Biotin-GAPDH antibody is human, mouse, rat, rabbit, and chicken reactive.
Positive Control 1:
Cat. No. 1201 - HeLa Cell Lysate
Predicted Molecular Weight:
Predicted: 37 kDa
Observed: 36 kDa
Purification:
Biotin-GAPDH antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
Biotin-GAPDH antibody is supplied in PBS containing 1% BSA and 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
Biotin-GAPDH antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Ncbi Official Symbol:
GAPDH
Additional Names:
Biotin-GAPDH, Glyceraldehyde-3-phosphate dehydrogenase, G3PDH, GAPD
Protein Accession Number:
NP_002037
Protein Gi Number:
7669492
Ncbi Gene Id Number:
2597
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD), an important energy-yielding step in carbohydrate metabolism. It also is involved in a number of cellular processes such as membrane fusion, phosphotransferase activity, DNA replication and repair, and nuclear RNA export (1). GAPDH also plays a role in different pathologies such as cancer progression, apoptosis, and neuronal diseases such as Alzheimer's and Huntington's disease (2). GAPDH is constitutively expressed at high levels in almost all tissues and cell lines making it ideal for use as a loading control marker in immunoblots.
Background Reference 1:
Sirover MA. New nuclear functions of the glycolytic protein, glyceraldehyde-3-phosphate dehydrogenase, in mammalian cells. J. Cell. Biochem. 2005; 95:45-52.
Background Reference 2:
Glyceraldehyde-3-phosphate dehydrogenase, apoptosis, and neurodegenerative diseases. Annu. Rev. Pharmacol. Toxicol. 2005; 45:269-90.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

3781-biotin

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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$65 Standard Overnight flat rate for as many products shipped within the United States.

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