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Western blot testing of human samples with GAPDH antibody at 0.5ug/ml.
Testing of mouse samples with GAPDH antibody at 0.5ug/ml.
Testing of human/mouse samples with GAPDH antibody at 0.5ug/ml.
IHC testing of FFPE human liver tissue with GAPDH antibody at 3ug/ml.
Western blot testing of human samples with GAPDH antibody at 0.5ug/ml.

GAPDH Antibody [GAP259]

CATALOG NUMBER: 33-017

Clonality:
Monoclonal
Tested Applications:
WB
Host Species:
Mouse
Species Reactivity:
Dog, Human, Mouse, Primate, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
Dog, Human, Mouse, Primate, Rat
Immunogen:
A recombinant protein fragment from C-terminal GAPDH was used as the immunogen.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
Western blot: 0.5-1 ug/ml
Provided assay concentrations are suggestions only, GAPDH antibody titration may be required for optimal results.
Purification:
Protein G Affinity Chromatography
Clonality:
Monoclonal
Isotype:
IgG1, kappa
Physical State:
Liquid
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.5 mg/mL
Storage Conditions:
Aliquot and Store at -20°C. Avoid freez-thaw cycles.
Ncbi Official Symbol:
GAPDH
Additional Names:
GAPDH Antibody: Glyceraldehyde-3-phosphate dehydrogenase, G3PDH, GAPD
Ncbi Gene Id Number:
2597
User Note:
Optimal dilutions for each application to be determined by the researcher
Background:
Due to its ubiquitous presence in mammalian cells, GAPDH is often used a loading control protein in western blot. GAPDH antibody is used to control for/demonstrate equivalent protein loading in different gel lanes, typically transfected vs mock or non transfected cell lysate.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

33-017

List Size:
100 ug

List Price:

$520.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

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