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Western blot analysis in K562 cell line lysates (35ug/lane).
Western blot analysis in K562 cell line lysates (35ug/lane).

GAR1 Antibody

CATALOG NUMBER: 60-507

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Zebrafish, Rat
Immunogen:
This GAR1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 86-114 amino acids from the Central region of human GAR1.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
22 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
GAR1
Additional Names:
H/ACA ribonucleoprotein complex subunit 1, Nucleolar protein family A member 1, snoRNP protein GAR1, GAR1, NOLA1
Protein Accession Number:
Q9NY12
Protein Gi Number:
51828015
Ncbi Gene Id Number:
54433
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene is a member of the H/ACA snoRNPs (smallnucleolar ribonucleoproteins) gene family. snoRNPs are involved invarious aspects of rRNA processing and modification and have beenclassified into two families: C/D and H/ACA. The H/ACA snoRNPs alsoinclude the DKC1, NOLA2 and NOLA3 proteins. These four H/ACA snoRNPproteins localize to the dense fibrillar components of nucleoli andto coiled (Cajal) bodies in the nucleus. Both 18S rRNA productionand rRNA pseudouridylation are impaired if any one of the fourproteins is depleted. These four H/ACA snoRNP proteins are alsocomponents of the telomerase complex. The encoded protein of thisgene contains two glycine- and arginine-rich domains and is relatedto Saccharomyces cerevisiae Gar1p. Two splice variants have beenfound for this gene.
Background Reference 1:
Kalsi, G., et al. Hum. Mol. Genet. 19(12):2497-2506(2010)
Background Reference 2:
Hamma, T., et al. J. Biol. Chem. 285(2):805-809(2010)
Background Reference 3:
Pigullo, S., et al. Pediatr Blood Cancer 52(3):376-378(2009)
Background Reference 4:
Vasan, R.S., et al. BMC Med. Genet.SUPPL 1, S2 (2007) :

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

60-507

List Size:
400 ul

List Price:

$495.00

Datasheet
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