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Western blot analysis of GAS in EL4 cell lysate in (A) the absence and (B) the presence of blocking peptide with GAS antibody at 1μg/mL.
Western blot analysis of GAS in EL4 cell lysate in (A) the absence and (B) the presence of blocking peptide with GAS antibody at 1μg/mL.
Trial Size

GAS Antibody

CATALOG NUMBER: 5437

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (93%), Rat: (93%)
Immunogen:
GAS antibody was raised against a 15 amino acid synthetic peptide from near the carboxy terminus of human GAS.
The immunogen is located within the last 50 amino acids of GAS.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
GAS antibody can be used for detection of GAS by Western blot at 1 μg/mL.
Antibody validated: Western Blot in mouse samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1287 - EL4 Cell Lysate
Purification:
GAS Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
GAS Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
GAS antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
PAGR1
Additional Names:
GAS Antibody: GAS, PA1, C16orf53, PAXIP1-associated glutamate-rich protein 1, PAXIP1-associated protein 1
Protein Accession Number:
NP_078792
Protein Gi Number:
13375654
Ncbi Gene Id Number:
79447
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
GAS Antibody: Steroid receptor co-activators (SRCs) were initially described as nuclear receptor transcription co-activators, but they have recently been determined to co-regulate transcription initiated by other transcription factors. GAS is a recently identified glutamate-rich protein that interacts with SRC1, but not GRIP1 or AIB1, the other two members of the SRC family. GAS can also interact with the alpha subunit of the estrogen receptor (ERalpha), but not other receptors such as the retinoic acid receptor a, suggesting the interaction between GAS and ERalpha is relatively specific. Depletion of GAS by RNA interference in MCF7 cells led to a decrease in the mRNA and protein levels of ER target genes such as pS2, c-Myc and cyclin D1, indicating the role of GAS in the regulation of ER target genes. GAS has also been found to associate with an SET1-like methyltransferase complex specific for H3K4 methylation, suggesting that GAS has multiple roles in transcriptional regulation.
Background Reference 1:
Xu J, Wu RC, and O'Malley BW. Normal and cancer-related functions of the p160 steroid receptor co-activator (SCR) family. Nat. Rev. Cancer2009; 9:615-30.
Background Reference 2:
Liang J, Zhang H, Zhang Y, et al. GAS, a new glutamate-rich protein, interacts differentially with SRCs and is involved in oestrogen receptor function. EMBO Rep.2009; 10:51-7.
Background Reference 3:
PTIP associates with MLL3- and MLL4-containing histone H3 lysine 4 methyltransferase complex. J. Biol. Chem.2007; 13:20395-406.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

5437

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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