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Antibody used in IHC on Human Liver cell lysates.
Antibody used in WB on Mouse liver, N2a at 1:500 (Lanes : Lane 1: 30ug mouse liver lysateLane 2: 30ug mouse N2a cell lysate).
Antibody used in WB on Human K562 cells at 1.0-2.0 ug/ml.
Antibody used in IHC on Human Liver cell lysates.

GATA2 Antibody

CATALOG NUMBER: 27-419

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Dog, Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Dog, Human, Mouse, Rat
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human GATA2.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
GATA2 antibody can be used for detection of GATA2 by ELISA at 1:1562500. GATA2 antibody can be used for detection of GATA2 by western blot at 1.0-2.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
Cat. No. 1204 - K562 Cell Lysate
Predicted Molecular Weight:
50 kDa
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store GATA2 antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
GATA2
Additional Names:
GATA2, DCML, NFE1B, MONOMAC
Protein Accession Number:
NP_116027
Protein Gi Number:
20070352
Ncbi Gene Id Number:
2624
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The GATA family of transcription factors, which contain zinc fingers in their DNA binding domain, have emerged as candidate regulators of gene expression in hematopoietic cells is essential for normal primitive and definitive erythropoiesis and is expressed at high levels in erythroid cells, mast cells, and megakaryocytes. GATA2 is expressed in hematopoietic progenitors, including early erythroid cells, mast cells, and megakaryocytes, and also in nonhematopoietic embryonic stem cells.
Background Reference 1:
Tsuzuki, S., et al., (2004) Mol. Cell. Biol. 24 (15), 6824-6836.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

27-419

List Size:
100 ul

List Price:

$473.00

Datasheet
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