Start Your Project

🛍️Explore our Promotions page to discover current deals, limited-time offers, and more!🌟

Western blot analysis of GATA3 in K562 cell lysate with GATA3 antibody at 1 μg/mL.
Immunohistochemistry of GATA3 in rat brain tissue with GATA3 antibody at 2.5 μg/mL.
Immunofluorescence of GATA3 in Rat Brain tissue with GATA3 antibody at 20 μg/mL.
Immunohistochemistry of GATA3 in mouse brain tissue with GATA3 antibody at 2.5 μg/ml.
Immunofluorescence of GATA3 in mouse brain tissue with GATA3 Antibody at 20 μg/mL.
Western blot analysis of GATA3 in K562 cell lysate with GATA3 antibody at 1 μg/mL.
Trial Size

GATA3 Antibody

CATALOG NUMBER: 5599

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (100%), Chicken: (100%), Pig: (72%)
Immunogen:
GATA3 antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of human GATA3.
The immunogen is located within amino acids 340 - 390 of GATA3.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
GATA3 antibody can be used for detection of GATA3 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse and rat samples and Immunofluorescence in mouse and rat samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1204 - K562 Cell Lysate
Predicted Molecular Weight:
Predicted: 49 kDa
Observed: 49 kDa
Purification:
GATA3 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
GATA3 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
GATA3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
GATA3
Additional Names:
GATA3 Antibody: HDR, HDRS, Trans-acting T-cell-specific transcription factor GATA-3, GATA-binding factor 3
Protein Accession Number:
NP_001002295
Protein Gi Number:
50541959
Ncbi Gene Id Number:
2625
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
GATA3 Antibody: GATA3 is a zinc finger transcription factor that was first recognized as a possible determinant of T cell development. Later studies demonstrated that it is expressed in trophoblast giant cells and regulates trophoblast-specific genes during development. Specifically, GATA3 is selectively expressed in the trophectoderm of the peri-implantation embryo and regulates CDX2, which is required for the transcriptional repression of the POU5F1/Oct4 and NANOG genes and is thus essential for the segregation of the inner cell mass and trophectoderm at the blastocyst stage. Decreased or null-expression expression of GATA3 has also been suggested to play a major role in the development and progression of luminal breast cancer.
Background Reference 1:
Ko LJ, Yamamoto M, Leonard MW, et al. Murine and human T-lymphocyte GATA-3 factors mediate transcription through a cis-regulatory element within the human T-cell receptor delta gene enhancer. Mol. Cell. Biol. 1991; 11:2778-84.
Background Reference 2:
Ma GT, Roth ME, Groskopf JC, et al. GATA-2 and GATA-3 regulate trophoblast-specific gene expression in vivo. Dev. 1997; 124:907-14.
Background Reference 3:
Home P, Ray S, Dutta D, et al. GATA3 is selectively expressed in the trophectoderm of peri-implantation embryo and directly regulates Cdx2 gene expression. J. Biol. Chem. 2009; 284:28729-37.
Background Reference 4:
Strumpf D, Mao CA, Yamanaka Y, et al. CDX2 is required for correct cell fate specification and differentiation of trophectoderm in the mouse blastocyst. Dev. 2005; 132:2093-102.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

5599

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

Customize your order

Bulk Pricing Available

Custom Formulations: azide free, endotoxin testing & endotoxin removal.

Pair your antibody with a Lysate, Peptide, Control, & /OR Secondary Antibody.

Contact our Customer Care Team for more information

Custom Antibody Services

Speak to one of our custom antibody specialists to discuss your next custom antibody project.

New & Featured Products

HIV-1 p24 Antibody [9D2F6] (HRP)
HIV-1 p24 Antibody [9D2F6] (HRP)

CATALOG NUMBER: PM-7369-HRP

HIV-1 p24 Antibody [9D2F6] (biotin)
HIV-1 p24 Antibody [9D2F6] (biotin)

CATALOG NUMBER: PM-7369-biotin

HIV-1 p24 Antibody [9D2F6]
HIV-1 p24 Antibody [9D2F6]

CATALOG NUMBER: PM-7369