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Antibody used in WB on Mouse tumor at: 1:1000 (Lanes: 1: 25 ug mouse tumor + shRNA1, 2: 25 ug mouse tumor + shRNA2, 3: 25 ug mouse tumor + shRNA3 4: 25 ug mouse tumor lysate).
Antibody used in WB on Human Jurkat 0.2-1 ug/ml.
Antibody used in WB on Mouse tumor at: 1:1000 (Lanes: 1: 25 ug mouse tumor + shRNA1, 2: 25 ug mouse tumor + shRNA2, 3: 25 ug mouse tumor + shRNA3 4: 25 ug mouse tumor lysate).

GATA4 Antibody

CATALOG NUMBER: 28-790

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Dog, Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Dog, Human, Mouse, Rat
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human GATA4.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
GATA4 antibody can be used for detection of GATA4 by ELISA at 1:1562500. GATA4 antibody can be used for detection of GATA4 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
Cat. No. 1205 - Jurkat Cell Lysate
Predicted Molecular Weight:
45 kDa
Purification:
Antibody is purified by peptide affinity chromatography method.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store GATA4 antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
GATA4
Additional Names:
GATA4, ASD2, VSD1, TACHD
Protein Accession Number:
NP_002043
Protein Gi Number:
33188461
Ncbi Gene Id Number:
2626
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
GATA4 is a member of the GATA family of zinc-finger transcription factors. Members of this family recognize the GATA motif which is present in the promoters of many genes. GATA4is thought to regulate genes involved in embryogenesis and in myocardial differentiation and function. Mutations in this gene have been associated with cardiac septal defects.This gene encodes a member of the GATA family of zinc-finger transcription factors. Members of this family recognize the GATA motif which is present in the promoters of many genes. This protein is thought to regulate genes involved in embryogenesis and in myocardial differentiation and function. Mutations in this gene have been associated with cardiac septal defects.
Background Reference 1:
Anttonen, M., et al., (2005) J. Clin. Endocrinol. Metab. 90 (12), 6529-6535.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

28-790

List Size:
100 ul

List Price:

$519.00

Datasheet
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