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Antibody used in WB on Human HepG2 at 0.2-1 ug/ml.
Antibody used in WB on Human HepG2 at 0.2-1 ug/ml.

GATAD2B Antibody

CATALOG NUMBER: 29-061

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Dog, Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Dog, Human, Mouse, Rat
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human GATAD2B.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
GATAD2B antibody can be used for detection of GATAD2B by ELISA at 1:312500. GATAD2B antibody can be used for detection of GATAD2B by western blot at 0.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
Cat. No. 1211 - HepG2 Cell Lysate
Predicted Molecular Weight:
65 kDa
Purification:
Antibody is purified by peptide affinity chromatography method.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store GATAD2B antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
GATAD2B
Additional Names:
GATAD2B, p68, MRD18, P66beta, RP11-216N14.6
Protein Accession Number:
NP_065750
Protein Gi Number:
21218438
Ncbi Gene Id Number:
57459
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Hp66beta (GATAD2B), previously identified as a component of the human MeCP1 complex, is a second member of a p66 gene family (hp66alpha and hp66beta). Both proteins interact with MBD2 and MBD3 functionally and biochemically. Both factors function in synergy, since knocking-down p66alpha affects the repressive function of p66beta and vice versa. Furthermore, confocal microscopy shows a nuclear colocalization of hp66alpha with hp66beta and MBD2 in a speckled pattern. These two highly related mammalian p66 proteins display overlapping functions and are involved in methylation dependent transcriptional repression.
Background Reference 1:
Beausoleil, S.A., (2004) Proc. Natl. Acad. Sci. U.S.A. 101 (33), 12130-12135.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

29-061

List Size:
100 ul

List Price:

$519.00

Datasheet
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