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Western blot analysis of extracts of various cell lines, using GATM antibody (22-341) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human liver using GATM Antibody (22-341) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using GATM Antibody (22-341) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse spleen using GATM antibody (22-341) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using GATM antibody (22-341) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of A549 cells using GATM antibody (22-341). Blue: DAPI for nuclear staining.
Western blot analysis of extracts of various cell lines, using GATM antibody (22-341) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.

GATM Antibody

CATALOG NUMBER: 22-341

Clonality:
Polyclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 124-423 of human GATM (NP_001473.1).
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:50 - 1:200
Positive Control 1:
Mouse kidney
Positive Control 2:
Rat kidney
Predicted Molecular Weight:
Observed: 43kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
GATM
Additional Names:
GATM, AGAT, AT, CCDS3
Ncbi Gene Id Number:
2628
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a mitochondrial enzyme that belongs to the amidinotransferase family. This enzyme is involved in creatine biosynthesis, whereby it catalyzes the transfer of a guanido group from L-arginine to glycine, resulting in guanidinoacetic acid, the immediate precursor of creatine. Mutations in this gene cause arginine:glycine amidinotransferase deficiency, an inborn error of creatine synthesis characterized by mental retardation, language impairment, and behavioral disorders.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

22-341

List Size:
100 uL

List Price:

$436.00

Datasheet
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