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Western blot analysis of GBE1 Antibody in HL-60 cell line lysates (35ug/lane).
Western blot analysis of GBE1 Antibody in mouse liver tissue lysates (35ug/lane)
Confocal immunofluorescent analysis of GBE1 Antibody with HepG2 cell followed by Alexa Fluor? 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).
Western blot analysis of GBE1 Antibody in HL-60 cell line lysates (35ug/lane).

GBE1 Antibody

CATALOG NUMBER: 63-981

Clonality:
Polyclonal
Tested Applications:
IF, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
This GBE1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 527-556 amino acids from the Central region of human GBE1.
Conjugate:
Unconjugated
Tested Applications:
IF, WB
Application Note:
For WB starting dilution is: 1:1000
For IF starting dilution is: 1:10~50
Predicted Molecular Weight:
80 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
GBE1
Additional Names:
4-alpha-glucan-branching enzyme, Brancher enzyme, Glycogen-branching enzyme, GBE1
Protein Accession Number:
Q04446
Protein Gi Number:
357529509
Ncbi Gene Id Number:
2632
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
GBE1 is a glycogen branching enzyme that catalyzes the transfer of alpha-1,4-linked glucosyl units from the outer end of a glycogen chain to an alpha-1,6 position on the same or a neighboring glycogen chain. Branching of the chains is essential to increase the solubility of the glycogen molecule and, consequently, in reducing the osmotic pressure within cells. Highest level of this enzyme are found in liver and muscle. Mutations in this gene are associated with glycogen storage disease IV (also known as Andersen's disease).
Background Reference 1:
Konstantinidou, A.E., et al. Placenta 29(4):378-381(2008)
Background Reference 2:
Massa, R., et al. Muscle Nerve 37(4):530-536(2008)
Background Reference 3:
Bruno, C., et al. Acta Myol 26(1):75-78(2007)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

63-981

List Size:
400 ul

List Price:

$495.00

Datasheet
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