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Western blot analysis of extracts of various cell lines, using GBP1 antibody (19-195) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 10s.
Immunohistochemistry of paraffin-embedded human liver cancer using GBP1 antibody (19-195) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of MCF7 cells using GBP1 antibody (19-195). Blue: DAPI for nuclear staining.
Western blot analysis of extracts of various cell lines, using GBP1 antibody (19-195) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.

GBP1 Antibody

CATALOG NUMBER: 19-195

Clonality:
Polyclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 383-592 of human GBP1 (NP_002044.2).
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:20 - 1:200
IF: 1:10 - 1:100
Positive Control 1:
5637
Positive Control 2:
Mouse liver
Positive Control 3:
Rat liver
Predicted Molecular Weight:
Observed: 68kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
GBP1
Additional Names:
GBP1, GBP-1, GTP-binding protein 1, Guanylate binding protein 1, HuGBP-1
Ncbi Gene Id Number:
2633
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Guanylate binding protein expression is induced by interferon. Guanylate binding proteins are characterized by their ability to specifically bind guanine nucleotides (GMP, GDP, and GTP) and are distinguished from the GTP-binding proteins by the presence of 2 binding motifs rather than 3.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

19-195

List Size:
100 uL

List Price:

$436.00

Out of stock

Datasheet
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