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45-659 (0.1ug/ml) staining of Jurkat cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
45-659 (3.75ug/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
45-659 (3.75ug/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
45-659 (0.1ug/ml) staining of Jurkat cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

GBX2 Antibody

CATALOG NUMBER: 45-659

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Pig, Cow
Immunogen:
The immunogen for this antibody is: C-QGKDESKVEDDPK
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 37kDa band observed in lysates of T-cell line Jurkat (calculated MW of 37.3kDa according to NP_001476.2). Recommended concentration: 0.1-0.3ug/ml. Primary incubation was 1 hour. Preliminary testing was unsuccessful on NIH-3T3 cell line for this particular batch.Immunohistochemistry:In paraffin embedded Human Placenta shows nuclear staining of selected trophoblasts. Recommended concentration: 4-8ug/ml. Paraffin embedded Human Brain (Cortex). Recommended concentration: 3.75ug/ml.
Positive Control 1:
Cat. No. 1205 - Jurkat Cell Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
GBX2
Additional Names:
GBX2, gastrulation brain homeobox 2 , gastrulation brain homeo box 2
Protein Accession Number:
NP_001476.2
Protein Gi Number:
45593144
Ncbi Gene Id Number:
2637
Background Reference 1:
Palmqvist L, Glover CH, Hsu L, Lu M, Bossen B, Piret JM, Humphries RK, Helgason CD. Correlation of murine embryonic stem cell gene expression profiles with functional measures of pluripotency. Stem Cells. 2005 May;23(5):663-80.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-659

List Size:
0.1 mg

List Price:

$495.00

Datasheet
Shipping Info

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