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Western blot analysis of GCAT using rabbit polyclonal GCAT Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the GCAT gene.
Western blot analysis of GCAT using rabbit polyclonal GCAT Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the GCAT gene.

GCAT Antibody

CATALOG NUMBER: 56-722

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Bovine
Immunogen:
This GCAT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 101-128 amino acids from the N-terminal region of human GCAT.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
45 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
GCAT
Additional Names:
2-amino-3-ketobutyrate coenzyme A ligase, mitochondrial, AKB ligase, Aminoacetone synthase, Glycine acetyltransferase, GCAT, KBL
Protein Accession Number:
O75600
Ncbi Gene Id Number:
23464
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The degradation of L-threonine to glycine consists of atwo-step biochemical pathway involving the enzymes L-threoninedehydrogenase and 2-amino-3-ketobutyrate coenzyme A ligase.L-Threonine is first converted into 2-amino-3-ketobutyrate byL-threonine dehydrogenase. This gene encodes the second enzyme inthis pathway, which then catalyzes the reaction between2-amino-3-ketobutyrate and coenzyme A to form glycine andacetyl-CoA. The encoded enzyme is considered a class IIpyridoxal-phosphate-dependent aminotransferase. Alternate splicingresults in multiple transcript variants. A pseudogene of this geneis found on chromosome 14.
Background Reference 1:
Jacquot, C., et al. Anticancer Res. 22(4):2229-2235(2002)
Background Reference 2:
Edgar, A.J., et al. Eur. J. Biochem. 267(6):1805-1812(2000)
Background Reference 3:
Tressel, T., et al. J. Biol. Chem. 261(35):16428-16437(1986)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

56-722

List Size:
400 ul

List Price:

$495.00

Datasheet
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