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Western blot analysis of GCAT antibody in mouse liver tissue lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human brain reacted with GCAT Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Flow cytometric analysis of HepG2 cells using GCAT Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of GCAT using rabbit polyclonal GCAT Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the GCAT gene.
Western blot analysis of GCAT antibody in mouse liver tissue lysates (35ug/lane)

GCAT Antibody

CATALOG NUMBER: 62-951

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Homology:
Predicted species reactivity based on immunogen sequence: Bovine
Immunogen:
This GCAT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 155-181 amino acids from the Central region of human GCAT.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
45 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
GCAT
Additional Names:
2-amino-3-ketobutyrate coenzyme A ligase, mitochondrial, AKB ligase, Aminoacetone synthase, Glycine acetyltransferase, GCAT, KBL
Protein Accession Number:
O75600
Ncbi Gene Id Number:
23464
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The degradation of L-threonine to glycine consists of a two-step biochemical pathway involving the enzymes L-threonine dehydrogenase and 2-amino-3-ketobutyrate coenzyme A ligase. L-Threonine is first converted into 2-amino-3-ketobutyrate by L-threonine dehydrogenase. GCAT is the second enzyme in this pathway, which then catalyzes the reaction between 2-amino-3-ketobutyrate and coenzyme A to form glycine and acetyl-CoA. The enzyme is considered a class II pyridoxal-phosphate-dependent aminotransferase.
Background Reference 1:
Edgar,A.J., Polak,J.M.Eur. J. Biochem. 267 (6), 1805-1812 (2000)
Background Reference 2:
Tressel,T., Thompson,R., J. Biol. Chem. 261 (35), 16428-16437 (1986)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

62-951

List Size:
400 ul

List Price:

$495.00

Datasheet
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