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Western blot analysis of extracts of various cell lines, using GCC2 antibody (15-215) at 1:3000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human colon using GCC2 antibody (15-215) at dilution of 1:150 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using GCC2 antibody (15-215) at dilution of 1:150 (40x lens).
Immunohistochemistry of paraffin-embedded human appendix using GCC2 antibody (15-215) at dilution of 1:150 (40x lens).
Immunohistochemistry of paraffin-embedded mouse spleen using GCC2 antibody (15-215) at dilution of 1:150 (40x lens).
Western blot analysis of extracts of various cell lines, using GCC2 antibody (15-215) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.

GCC2 Antibody

CATALOG NUMBER: 15-215

Clonality:
Polyclonal
Tested Applications:
IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human GCC2 (NP_852118.1).
Conjugate:
Unconjugated
Tested Applications:
IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
Positive Control 1:
HeLa
Positive Control 2:
LO2
Positive Control 3:
22Rv1
Positive Control 4:
Raji
Positive Control 5:
SGC-7901
Positive Control 6:
293T
Predicted Molecular Weight:
Observed: 196kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
GCC2
Additional Names:
GCC2, GRIP and coiled-coil domain containing 2, GCC185, KIAA0336, CLL-associated antigen KW-11, CTCL tumor antigen se1-1, GRIP and coiled-coil domain-containing 2, GRIP coiled-coil protein GCC185, (Ran-binding protein 2-like 4), RANBP2L4
Ncbi Gene Id Number:
9648
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene is a peripheral membrane protein localized to the trans-Golgi network. It is sensitive to brefeldin A. This encoded protein contains a GRIP domain which is thought to be used in targeting. Alternative splicing results in multiple transcript variants.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

15-215

List Size:
100 uL

List Price:

$436.00

Datasheet
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