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Western blot analysis in MDA-MB231 cell line lysates (35ug/lane).
Confocal immunofluorescent analysis of GCDFP-15 Antibody with MDA-MB231 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
GCDFP-15 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western blot analysis in MDA-MB231 cell line lysates (35ug/lane).

GCDFP-15 Antibody

CATALOG NUMBER: 55-647

Clonality:
Polyclonal
Tested Applications:
IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This GCDFP-15 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 77-106 amino acids from the C-terminal region of human GCDFP-15.
Conjugate:
Unconjugated
Tested Applications:
IF, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IF starting dilution is: 1:10~50
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
17 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
PIP
Additional Names:
Prolactin-inducible protein, Gross cystic disease fluid protein 15, GCDFP-15, Prolactin-induced protein, Secretory actin-binding protein, SABP, gp17, PIP, GCDFP15, GPIP4
Protein Accession Number:
P12273
Protein Gi Number:
134170
Ncbi Gene Id Number:
5304
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Catalyzes the addition of fucose in alpha 1-6 linkage to the first GlcNAc residue, next to the peptide chains in N-glycans.
Background Reference 1:
Bryborn, M., et al. Allergy 65(2):220-228(2010)
Background Reference 2:
Wang, L.J., et al. Appl. Immunohistochem. Mol. Morphol. 17(6):505-511(2009)
Background Reference 3:
Yan, Z., et al. Diagn. Cytopathol. 37(7):475-478(2009)
Background Reference 4:
Debily, M.A., et al. PLoS ONE(3), E4696 (2009) :

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

55-647

List Size:
400 ul

List Price:

$495.00

Datasheet
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